Part:BBa_K277039

3L.3_23.B1.13

3L.3_23.B1.13 is 869 bases long and is cloned into the pGem-T vector.

3L.3_23.B1.13 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.B1.13 is a constituent of 3L.3_23.B1 (along with 3L.3_23.B1.01, 3L.3_23.B1.02, 3L.3_23.B1.03, 3L.3_23.B1.04, 3L.3_23.B1.05, 3L.3_23.B1.06, 3L.3_23.B1.07, 3L.3_23.B1.08, 3L.3_23.B1.09, 3L.3_23.B1.10, 3L.3_23.B1.11, 3L.3_23.B1.12, and 3L.3_23.B1.14.)

This part contains at least part of the following features (positions offset from first base of sequence):

kind and name offset notes

gene YCL050C (125..+1090) Diadenosine 5'%2C5-P1%2CP4-tetraphosphate phosphorylase I (AP4A phosphorylase)%2C involved in catabolism of bis(5'-nucleosidyl) tetraphosphates%3B has similarity to Apa2p

reverse_primer YCL050C_tagr1v1 (620..647)

mutation_affecting_coding_sequence YCL050C_re_remove_MmeI (680..691) removal of MmeI

forward_primer YCL050C_tagf1v1 (293..320)

loxP_site loxPsym_YCL050C (88..121)

Sequence (the first 869 bases correspond to coordinates 27983..28851 in synthetic chromosome yeast_chr3_3_23)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]